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The Fluo-100 is a dual-channel fluorometer, it provides high-sensitive fluorescent detection when quantifying nucleic acids and proteins, simple and cost to operate Applications: 1. The sample is scarce and difficult in processing 2. The low quantity of DNA, RNA, or protein after extraction 3. The sample will be used in the expensive experiments: qPCR, PCR cloning, transfection, the next generation sequencing and etc.
Fluorometer technical background:
Features:
1. Simple – 4.3 inch touch screen, small and easy to use.
2. Easy measurement within 3 sec for DNA, RNA, and protein.
3. High sensitivity – Lowest DNA detection limit is 0.5pg/ul.
4. Two optical channels – Equipped with two fluorescence channels for nucleic acid, protein quantitation in one detection.
5. Linear Dynamic Range – Five orders of magnitude.
6. Opening system – You can match the instrument with the reagent you choose.
7. It can save at most 1,000 data and output data by USB port.
8. Adapter: 0.5ml qPCR tube adapter 0.2ml qPCR tube adapter.
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Light source
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LED
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Dynamic range
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Five orders of magnitude
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Linear Dynamic Range
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R2>0.995
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Detector
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Photodiode
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Repeatability
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<1.5%
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Stability
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<1.5%
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Sensitivity
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dsDNA: 0.5ng/ml
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Measurement speed
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3s (Once)
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Dimension (W×D×H)mm
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194×155×72.5 mm
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Weight (kg)
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0.4Kg
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Ordering information
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Code
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Description
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Code
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Description
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AS-18010-00
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Fluo-100A Fluorometer, DC12V 1A
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RS-FL0101-M RS-FL0101-L |
High-sensitivity dsDNA Quantitation kits (200 assays) High-sensitivity dsDNA Quantitation kits (1000 assays) |
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AS-18020-00
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Fluo-100B Fluorometer, DC12V 1A
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AS-18030-00
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Fluo-100C Fluorometer, DC12V 1A
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RS-FL0102-M RS-FL0102-L |
Broad-range dsDNA Quantitation kits (200 assays) Broad-range dsDNA Quantitation kits (1000 assays) |
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AS-18011-01
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Adapter for 0.5ml qPCR tube (Standard)
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AS-18011-02
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Adapter for 0.2ml qPCR tube (Options)
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RS-FL0103-S
RS-FL0103-M RS-FL0103-L |
High-sensitivity ssDNA Quantitation kits (100 assays)
High-sensitivity ssDNA Quantitation kits (200 assays) High-sensitivity ssDNA Quantitation kits (1000 assays) |
Fluo-100 Simple, easy to use and applications:
The Fluo-100 is a dual-channel fluorometer, it provides high-sensitive fluorescent detection when quantifying nucleic acids and proteins, simple and cost to operate.
Applications:
1. The sample is scarce and difficult in processing.
2. The low quantity of DNA, RNA, or protein after extraction.
3. The sample will be used in the expensive experiments: qPCR, PCR cloning, transfection, the next generation sequencing and etc.
Fluo-100 Quantitation kit operation:
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Two detection models of Fluo-100
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Code
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Light
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Excitation Filters
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Emission Filters
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UV
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365±20nm
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420-480nm (60nm)
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Blue
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460±20nm
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525-570nm (45nm)
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Blue
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460±20nm
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525-570nm (45nm)
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Red
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625±10nm
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670-725nm (55nm)
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Blue
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460±20nm
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525-570nm (45nm)
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Green
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525±20nm
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575-640nm (65nm)
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Fluo-100 Quantitation kit:
High sensitivity of dsDNA Quantitation Kit: 0.2--100ng
High sensitivity of ssDNA Quantitation Kit: 1--200ng
Broad range of dsDNA Quantitation Kit: 10--1000ng
Protein Quantitation Kit is researching.
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Application of different fluorescence channel
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Channel
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Excitation Filters
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Normal kits
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Applications
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UV channel
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365±20nm
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Hoechst 33258, 4-MU,
EnZCheK Caspase |
Nuclear acid quantification, Plant GUS reporter gene detection, Poptosis detection
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PicoGreen®, oligreen,RiboGreen®,
GFP, Protein, Fluorescein, Quant-iT™ |
dsDNA, ssDNA, RNA quantification, GFP gene detection, Fluorescein detection, Protein detection
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Rhodamine, Cy3, RFP Vybrant Cytotoxicity
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Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, Cell activity poison detection
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Red channel
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625±10nm
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Cy5, Quant-iT RNA
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Cy-5 fluorescence labeling detection, RNA quantification
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